BP Fluor 532 maleimide

BP Fluor 532 maleimide is a photostable, bright yellow fluorescent dye that is not sensitive to pH, emitting light across a range of pH 4 to pH 10. Its excitation is well-suited for second harmonic Nd:YAG laser lines. BP Fluor 532 can bind with various antibodies, peptides, proteins, tracers, and amplification substrates and is commonly used in imaging and flow cytometry to produce stable signals. Maleimide is the most frequently employed thiol-reactive group for linking dyes to thiol groups on proteins, oligonucleotide phosphorothioates, or low-molecular-weight ligands. It reacts with reduced thiols (sulfhydryl, -SH) specifically and efficiently at pH 6.5 to 7.5, forming stable thioether bonds. The resulting conjugates exhibit brighter fluorescence and greater photostability than those of many other spectrally similar fluorophores.