Nitric oxide production-IN-2

TLR4/JNK/NF-?B-IN-1 (Racemic-11k) is an inhibitor of TLR4, JNK, and NF-?B. It suppresses NO production in LPS-stimulated RAW264.7 cells with an IC50 of 23.2 µM. By inhibiting TLR4 expression and reducing JNK phosphorylation, TLR4/JNK/NF-?B-IN-1 prevents NF-?B activation. This leads to a decrease in the transcription of inflammation-related genes, reducing the expression of iNOS and COX-2, and the production of inflammatory mediators such as NO, PGE2, and TNF-?, thereby exhibiting anti-inflammatory activity. TLR4/JNK/NF-?B-IN-1 holds potential in the study of inflammatory diseases, including rheumatoid arthritis and various other inflammatory conditions.