4-Methyl-7- (2-nitrophenoxy) -2H-chromen-2-one

Compound 2f, chemically known as 4-Methyl-7- (2-nitrophenoxy) -2H-chromen-2-one, is a selenium alcohol fluorescent probe designed on the nucleophilic aromatic substitution mechanism. It exhibits selective recognition of selenols in neutral aqueous solutions without significant interference from biological thiols, amines, or alcohols. This compound is utilized for quantifying selenium content in selenoproteins and imaging the activity of endogenous selenols in living cells.