Anti-HA Antibody

Rabbit Polyclonal Antibody specific to HA

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-HA Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

Immunogen

HA (YPYDVPDYA) conjugated to KLH.

Target

Clonality

Polyclonal

Type

Antibody

Applications

WB, IP, ICC, ELISA

Field of Research

Epitope Tags

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500 These are recommended dilutions. Endusers should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at 2-8°C.

Specificity

Antibody was affinity-purified on the peptide.

Formulation

PBS, pH 7.2, 0.09% sodium azide.

Buffer pH

pH 7.2

Target Background

The HA epitope tag is derived from the HA-molecule corresponding to amino acids YPYDVPDYA of human influenza hemagglutinin. It is frequently used as an epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.