Drug Affinity Responsive Target Stability (DARTS) Assay Kit

Drug Affinity Responsive Target Stability (DARTS) Assay Kit, based on the conventioanl DARTS technology, provides a method to verify the direct binding between small-molecule drugs and target proteins without the need for compound modification. First proposed by Lomenick et al. in 2009, this technique has become a sensitive, reliable, and easy-to-use tool for drug target identification and mechanism-of-action studies. This kit provides the key buffers and protease digestion systems required for DARTS experiments, enabling efficient and sensitive detection of compound–target protein interactions. The method requires no compound labeling or modification, features a simple workflow and intuitive results, and can be combined with Western blotting, LC-MS/MS, or proteomics analysis. It offers an efficient and reliable experimental solution for drug discovery, target identification, and mechanism research.