His Tag Agarose NTA-Nickel

His Tag Agarose NTA-Nickel (NTA-Ni), a 4% highly cross-linked agarose, consists of nitrilotriacetic acid (NTA) chelator-activated agarose beads that subsequently charged with divalent nickel (Ni2+) ions by four coordination sites. The product is suitable for purifying His-tagged proteins—such as 6xHis-tag proteins—expressed in various systems, including E. coli, yeast, insect cells, and mammalian expression systems.