7-AAD Viability Staining BufferCatalog #
7-Aminoactinomycin D (7-AAD) is a fluorescent chemical compound with a strong affinity for DNA. It is used as a fluorescent marker for DNA in fluorescence microscopy and flow cytometry. It intercalates in double-stranded DNA, with a high affinity for GC-rich regions, making it useful for chromosome banding studies. With an absorption maximum at 546 nm, 7-AAD is efficiently excited using a 543 nm helium-neon laser; it can also be excited with somewhat lower efficiency using a 488 nm or 514 nm argon laser lines.
Product Specifications
Applications
Flow cytometry
Buffer
In PBS (pH7.2), 0.09% NaN3
Storage Conditions
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Usage
This reagent is used to analyze cell cycle by flow cytometry. Itneed notdilution. Use 5 μL /testand incubate for 5 minutes at room temperature before analysis.
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