TMBSubstrate (2-component) for ELISA (TMB Substrate A and TMB Substrate B)

The TMB Substrate contains 110 ml TMB Substrate A and 110 ml TMB Substrate B, sufficient for HRP conjugated ELISA. TMB Substrate A is formulated with hydrogen peroxide; TMB Substrate B is formulated with 3, 3', 5, 5' tetramethyl benzidine (TMB) . TMB is the substrate of horseradish peroxidase (HRP) . The substrate can be catalyzed with peroxidase to produce a pale blue color. The color is positively correlated with HRP activity, which can be used to detect HRP markers.