Cell Counting Kit-8, CCK8 Cell Viability Assay Kit

CCK8 is WST-8 based cell viability assay kit which was widely used for cell proliferation and cytotoxicity assay. WST-8 (2- (2-methoxy-4-nitrophenyl) -3- (4-nitrophenyl) -5- (2,4-disulfophenyl) -2H-tetrazolium) is reduced by dehydrogenases of cell mitochondria, combined with PMS electron mediator, and yield a water-soluble formazan. The faster of the cell proliferation, the deeper of the color. The greater of the cell cytotoxicity, the lighter of the color. WST-8 is better than MTT, XTT, MTS. WST-1 and in particular WST-8 are advantageous over MTT in that they are reduced outside cells, combined with PMS electron mediator, and yield a water-soluble formazan. Finally, WST assays can be read directly (unlike MTT that needs a solubilization step), give a more effective signal than MTT, and decrease toxicity to cells (unlike cell-permeable MTT, and its insoluble formazan that accumulate inside cells) . WST-8 is more stable and sensitive than XTT and MTS, which can ensure the stable and high sensitive results. In addition, compare to WST-1, WST-8 is also more sensitive and stable than WST-1, and can be easily dissolved. This kit can be used for cytokines induced cell proliferation assay, anticarcinogen induced cell cytotoxicity assay or drug induced cell growth inhibition assay. This kit is a one-bottle solution, without premixing any components. All assays can be proceeded in one 96-well plate without wash, cell collecting and additional formazan dissolution. For this, it can be used to detect samples in bulk. In addition, since WST-8 has no toxicity to cells, after coloration, the plate can be read repeatedly, end user have the flexible detection time to choose the optimal one further in this way. Phenol red and serum do not influence the performance of this kit. This kit can be enough for 500 assays.