MTT Cell Proliferation and Cytotoxicity Assay Kit

Since cell viability and numbers were positively correlated, MTT Cell Proliferation and Cytotoxicity Assay Kit was used widely to detect the cell proliferation and cytotoxicity. MTT [3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2-H-tetrazolium bromide] can be reverted to the purple crystal Formazan by the dehydrogenase of mitochondria, and the Formazan can be solved thoroughly by a certain solvent. Read the O.D. Value at 570 nm, the O.D. Value is more higher, the cell proliferation is more sooner; conversely, if the O.D. Value is more lower, the cell cytotoxicity is more bigger. With the unique Formazan diluent buffer, it is not necessary to remove the intrinsic cell culture medium, but add the kit component Formazan diluent buffer directly, in this way, the unnecessary assay error can be avoided. With the advantages of low background, high sensitivity, easy operation and wide linearity range, this kit can increase the reliability of the assay.