WB / Antibody Stripping Buffer (Alkaline)

WB Stripping Buffer is usedforthe recycle of deproteinized membranein WB.In WB, after completingthe conjugation of theprimary andsecondaryantibodies as wellasthelater chemiluminescence, sometimes end user has todetecttheproteins withstable expression level like Actin and Tubulin, etc.forreference, ortestother proteinsfor comparison. But for this reagent, through dissociatingthe conjugationof primary, secondaryantibodies and antigens, it removes primary and secondary antibodies from the membrane, then end user can reuse the same membrane to detect other proteins. Thus, compare with running a fresh SDS-PAGE gel, this reagent helps to eliminate the loading error and makes the experiment more comparable and accurate. For excluding thefrequently-used β-Mercaptoethanol, this reagent is nontoxic, tasteless andharmless, can be used at room temperature. This reagent is enough for at least 5 times WB detections on the same membrane, and the whole detection can be completedwithin15-30 min. Withtheunique component, it is efficient, andhas no harm tothe proteins.