BCA ProteinAssayKit

BCA (bicinchoninic acid) is a perfectproteinassaymethod, it serves the purpose of the Folin reagent in the Lowry assay, namely to react with complexes between copper ions and peptide bonds to produce a purple end product. This method is favored by professionals for itshighsensitivity, stability, reliability, andsmallvariation coefficient on different kinds of protein detections. Determination of the protein concentration with BCA is notinfluenced by chemical substances in majority of the samples.For example, intissue andcell lysate experiments, thetest results were not affected by commondetergents (SDS, Triton X-100, Tween), but will be influenced bychelant (EDTA, EGTA), deoxidizer (DTT, mercaptoethanol) and lipid. So, in the assay, if the background of the sample diluent buffer or lysis buffer is high, end user can try to use Bradford Protein Assay Kit (Catalog # BWR1027) . This kit can work well here.