Nuclear and Cytoplasmic Protein Extraction Kit

Nuclear and Cytoplasmic Protein Extraction Kitis designed to quickly prepare highly enriched fractions of cytoplasmic and nuclear proteins from eukaryotic samples, such as cultured cells and fresh tissues. This kitprovides a simple and convenient method for extracting nuclear and cytoplasmic protein. The user cancomplete the separation of nuclear and cytoplasmic proteinwithin about 90 min. Extracted proteins can be directly apply for downstream reactions, such as Western, EMSA, footprinting, reporter gene detection, and measurement of enzyme activity. Through Cytoplasmic Protein Extraction ReagentAandB, this kit breaks cell membrane and releasesproteins forcellburstunder hypotonic condition, then centrifuge for collection of the nucleoli. At last, extract the nuclear proteins by hyperhalineNuclear Protein Extraction Reagent. The kit can be usedfor50samples (60mg/sample, or 2x106Helacells) .User can adjust the volume of reagent according to different sample volumes.