RIPA Lysis Buffer (Medium)

ThisRIPA Lysis Bufferis a kind oftraditionalcelland tissue lysisbuffer.The end protein / sample lysed by this buffer can be used forWesternBlot and Immunoprecipitation. RIPA (Radio Immunoprecipitation Assay) Lysis Buffer has differentformulas, and according to its lysis strength, it can be roughly classified into three categories: strong, medium and weak. RIPA Lysis Buffer (Medium) contains:50mM Tris (pH 7.4),150mM NaCl,1%NP-40,1% sodium deoxycholate,0.1% SDS, and different kinds of inhibitors (sodium orthovanadate, sodium fluoride, EDTAandleupeptin), which can effectivelyinhibitthe protein degradation. The concentration of the protein which lysed by this buffer can be determined by our BCA Protein Assay Kits (Catalog # BWR1023, BWR1024, BWR1025, BWR1026) . For thehigh concentrationofdetergent, it is not recommended to useBradfordProtein Assay Kit.