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Monoclonal Anti-α-Tubulin

Product Specifications

Background

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8.

Host

Mouse

Reactivity

Human, mouse, rat, dog, rabbit, pig, cat, chicken, sea urchin, chlamydomonas

Immunogen

Purified humanα-Tubulinprotein.

Applications

Western Blot (1:100-1000) ; Immunoprecipitation, IP (1:100) ; Immunofluorescence, IF (1:500) ; Immunohistochemistry, IHC (1:500)

Form

Liquid

References & Citations

1. Williams RC Jr, Shah C, Sackett D (November 1999) . "Separation of tubulin isoforms by isoelectric focusing in immobilized pH gradient gels". Anal Biochem 275 (2) : 265–7.

Storage Conditions

Store at -20°C for 1 year. Avoid repeated freeze and thaw cycles.

Specificity

Recognize the C-terminal ofα-Tubulin.

Isotype

IgG1

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