Polyclonal Anti- GAPDH antibody
Product Specifications
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.
Host
Rabbit
Reactivity
Human, mouse, rat, pig, bovine, goat, monkey, chicken
Immunogen
A synthesized peptide derived from human GAPDH.
Applications
Western Blot (1:1,500-1:15,000) ; Immunohistochemistry, IHC (1:20-1:100) ; Immunocytochemistry, ICC/IF (1:100)
Form
Liquid
Buffer
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
References & Citations
1. Meyer-Siegler, K., Mauro, D. J., Seal, G., Wurzer, J., deRiel, J. K., Sirover, M. A. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase. Proc. Nat. Acad. Sci. 88: 8460-8464, 1991. 2. Zheng L, Roeder RG, Luo Y (2003) . "S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component". Cell 114 (2) : 255–66. 3. A. Tarze, A. Deniaud, M. Le Bras, E. Maillier, D. Molle, N. Larochette, N. Zamzami, G. Jan, G. Kroemer, and C. Brenner (2007) . "GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization". Oncogene 26 (18) : 2606–2620.
Storage Conditions
Store at -20°C for 1 year. Avoid repeated freeze and thaw cycles.
Specificity
Detect the endogenous levels of total GAPDH.
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