Monoclonal Anti-GAPDH antibody
Product Specifications
Background
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.
Host
Mouse
Reactivity
Human, monkey
Immunogen
A synthetic peptide (conjugated to KLH) corresponding to the C-terminal of human GAPDH.
Applications
Western Blot (1:5000) ; Immunofluorescence, IF (1:500) ; ELISA (1:1000-2000)
Form
Liquid
References & Citations
1. Meyer-Siegler, K., Mauro, D. J., Seal, G., Wurzer, J., deRiel, J. K., Sirover, M. A. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glyceraldehyde-3-phosphate dehydrogenase. Proc. Nat. Acad. Sci. 88: 8460-8464, 1991. 2. Zheng L, Roeder RG, Luo Y (2003) . "S phase activation of the histone H2B promoter by OCA-S, a coactivator complex that contains GAPDH as a key component". Cell 114 (2) : 255–66. 3. A. Tarze, A. Deniaud, M. Le Bras, E. Maillier, D. Molle, N. Larochette, N. Zamzami, G. Jan, G. Kroemer, and C. Brenner (2007) . "GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilization". Oncogene 26 (18) : 2606–2620.
Storage Conditions
Store at -20°C for 1 year. Avoid repeated freeze and thaw cycles.
Specificity
Detect the endogenous levels of total GAPDH protein.
Isotype
IgG1
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