Monoclonal Anti-T7 Tag

Product Specifications

Background

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene 10. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems.

Host

Mouse

Reactivity

All

Immunogen

A synthetic peptideMASMTGGQQMG conjugated to KLH.

Applications

Western Blot (1:1000-10000) ; Immunofluorescence, IF (1:100-1:1000) ; Immunoprecipitation, IP (1:100-1:1000)

Form

Liquid

Buffer

In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

References & Citations

1. Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol. 1986 May 5;189 (1) :113-30