Monoclonal Anti-Myc Tag (HRP Conjugated)
Product Specifications
Background
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, over expressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da) . It can be fused to the C-terminus and the N-terminus of a protein.
Host
Mouse
Reactivity
All
Immunogen
A synthetic peptide (conjugated to KLH) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL) .
Applications
Western Blot (1:5000)
Form
Liquid
Storage Conditions
Store at -20°C for 1 year. Or aliquot and store at -80°C for long term. Avoid repeated freeze and thaw cycles.
Specificity
Detect over-expressed or recombinant proteins containing the Myc epitope tag.
Isotype
IgG1
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