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Monoclonal Anti-mCherry Tag

Product Specifications

Background

MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components.mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t0.5of 15 minutes, allowing it to be visualised soon after translation.

Host

Mouse

Reactivity

All

Immunogen

Recombinant mCherry proteinwith full length.

Applications

Western Blot (1:1000-10000)

Form

Liquid

Buffer

In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.

References & Citations

1. Shaner, Nathan C; Campbell, Robert E; Steinbach, Paul A; Giepmans, Ben N G; Palmer, Amy E; Tsien, Roger Y (2004) . "Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. Red fluorescent protein". Nature Biotechnology 22 (12) : 1567–72.

Storage Conditions

Store at -20°C for 1 year. Or aliquot and store at -80°C for long term. Avoid repeated freeze and thaw cycles.

Specificity

Detect the expression level of mCherry tag fusion proteins.

Isotype

IgG1

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