Monoclonal Anti-mCherry Tag
Product Specifications
Background
MCherry is a fluorophore (a fluorescent molecule) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cells components.mCherry is a monomeric fluorescent construct which absorbs at 587 nm and emits at 610 nm. It is resistant to photobleaching and is stable. It matures quickly, with a t0.5of 15 minutes, allowing it to be visualised soon after translation.
Host
Mouse
Reactivity
All
Immunogen
Recombinant mCherry proteinwith full length.
Applications
Western Blot (1:1000-10000)
Form
Liquid
Buffer
In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
References & Citations
1. Shaner, Nathan C; Campbell, Robert E; Steinbach, Paul A; Giepmans, Ben N G; Palmer, Amy E; Tsien, Roger Y (2004) . "Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. Red fluorescent protein". Nature Biotechnology 22 (12) : 1567–72.
Storage Conditions
Store at -20°C for 1 year. Or aliquot and store at -80°C for long term. Avoid repeated freeze and thaw cycles.
Specificity
Detect the expression level of mCherry tag fusion proteins.
Isotype
IgG1
Curated Selection
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