Monoclonal Anti-MBP Tag
Product Specifications
Background
Maltose-Binding Protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It has an approximate molecular mass of 42.5 kilodaltons. MBP is used to increase the solubility of recombinant proteins expressed in E. coli. In these systems, the protein of interest is often expressed as a MBP-fusion protein, preventing aggregation of the protein of interest. In addition, MBP can itself be used as an affinity tag for purification of recombinant proteins. The fusion protein binds to amylose columns while all other proteins flow through. The MBP-protein fusion can be purified by eluting the column with maltose.
Host
Mouse
Reactivity
All
Immunogen
Recombinant MBP protein with full length.
Applications
Western Blot (1:1000-10000)
Form
Liquid
Buffer
In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
References & Citations
1. Claude V. Maina, Paul D. Riggs, Andres G. Grandea III, Barton E. Slatko, Laurie S. Moran, John A. Tagliamonte, Larry A. McReynolds, di Guan Chu. "An Escherichia coli vector to express and purify foreign proteins by fusion to and separation from maltose-binding protein" Gene Volume 74, Issue 2, 30 December 1988, Pages 365–373.
Storage Conditions
Store at 2-8°C for 1 month. Or aliquot and store at -20°C or below for long term. Avoid repeated freeze and thaw cycles.
Isotype
IgG1
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