Monoclonal Anti-HPC4 antibody
Product Specifications
Background
Protein C, also known as autoprothrombin IIA and blood coagulation factor XIV, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death, and maintaining the permeability of blood vessel walls in humans and other animals. The zymogenic form of protein C is a vitamin K-dependent glycoprotein that circulates in blood plasma. Its structure is that of a two-chain polypeptide consisting of a light chain and a heavy chain connected by a disulfide bond. Protein C Tag (HPC4) is a 12 amino acid sequence EDQVDPRLIDGK, which encodes residues 6 through 17 of the heavy chain of the protein.Protein C Tag Antibody is a useful tool in analysis of Protein C fusion proteins.
Host
Mouse
Reactivity
All
Immunogen
A synthetic peptide CEDQVDPRLIDGKconjugated to KLH.
Applications
Western Blot (1:1000-10000)
Form
Liquid
Buffer
In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
References & Citations
1. Mather T, Oganessyan V, Hof P, Huber R, Foundling S, Esmon C, Bode W (December 1996) . "The 2.8 Å crystal structure of Gla-domainless activated protein C". EMBO J. 15 (24) : 6822–31. 2. Foster DC, Yoshitake S, Davie EW (July 1985) . "The nucleotide sequence of the gene for human protein C". Proc. Natl. Acad. Sci. U.S.A. 82 (14) : 4673–7.
Storage Conditions
Store at -20°C for 1 year. Or aliquot and store at -80°C for long term. Avoid repeated freeze and thaw cycles.
Specificity
Recognize C-terminal, internal and N-terminal Protein C epitope (EDQVDPRLIDGK) tagged proteins.
Isotype
IgG1
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