Monoclonal Anti-Flag/ DYKDDDDKTag
Product Specifications
Background
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. The peptide sequence of the FLAG-tag is: N-DYKDDDDK-C (1012 Da) . It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein.
Host
Mouse
Reactivity
All
Immunogen
A synthetic peptideDYKDDDDKconjugated to KLH.
Applications
Western Blot (1:1000-10000) ; Immunofluorescence, IF (1:200-1000) ; Immunoprecipitation, IP (1:100-500)
Form
Liquid
Buffer
In PBS (pH7.4), 50% Glycerol and 0.02% Sodium Azide.
Storage Conditions
Store at 2-8°C for 1 month. Or aliquot and store at -20°C or below for long term. Avoid repeated freeze and thaw cycles.
Isotype
IgG1
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