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Monoclonal Anti- Neuron-specific class IIIβ-tubulin, β3-tubulin antibody

Selected Variant

BMA1045

Size: 100 µg

Product Specifications

Background

Tubulin is one of several members of a small family ofHYPERLINK "http://en.wikipedia.org/wiki/Globular_protein" \o "Globular protein"globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, each has aHYPERLINK "http://en.wikipedia.org/wiki/Molecular_weight" \o "Molecular weight"molecular weightof approximately 55 kHYPERLINK "http://en.wikipedia.org/wiki/Dalton_ (unit) " \o "Dalton (unit) "Da.Neuron-specific class III β-Tubulin, also called TUBB3, is aHYPERLINK "http://en.wikipedia.org/wiki/Protein" \o "Protein"proteinthat in humans is encoded by the TUBB3HYPERLINK "http://en.wikipedia.org/wiki/Gene" \o "Gene"genewhich maps to chromosome 16q24.3.HYPERLINK "http://en.wikipedia.org/wiki/TUBB3" \l "cite_note-pmid9473684-0"[1]It was significantly expressed in fibroblasts. And it has an important function in antimicrotubule drug resistance.[2]

Host

Mouse

Reactivity

Human, mouse, rat

Immunogen

A synthetic peptide (conjugated with KLH) corresponding to the N-terminal ofβ3-tubulin.

Clone

T28

Applications

Western Blot, WB (1:500-1:2, 000) ; Immunohistochemistry, IHC-P (1:50-1:100) ; Immunocytochemistry, ICC (1:200-1:500) ; Flow Cytometry, Flow-Cyt (1:20-1:50)

Purification

Peptide affinity purified

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

References & Citations

Venter JC (Jun 1993) . "Identification of new human receptor and transporter genes by high throughput cDNA (EST) sequencing".J Pharm Pharmacol. 45 Suppl 1: 355–60. Ranganathan S, Dexter DW, Benetatos CA, Hudes GR (Mar 1998) . "Cloning and sequencing of human betaIII-tubulin cDNA: induction of betaIII isotype in human prostate carcinoma cells by acute exposure to antimicrotubule agents".Biochim Biophys Acta1395 (2) : 237–45.

Storage Conditions

Store at 4°C after thawing (1 week). Aliquot and store at -20°C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Isotype

IgG2a

Price

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