Monoclonal Anti- Tri-Methyl-Histone H3 / Lys9 antibody

Product Specifications

Background

Histones are the basic nuclear proteins responsible for the nucleosome structure within the chromosomal fiber in eukaryotes. Five classes of histone genes have been reported , Histone H3 is one of them.[1]Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones.It is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.[2]

Host

Mouse

Reactivity

Human, mouse, rat

Immunogen

A synthetic peptide (conjugated with KLH) corresponding to the region of Lys9.

Clone

S49-8

Applications

Western Blot, WB (1:2, 000-1:5, 000) ; Immunohistochemistry, IHC-P (1:100) ; Immunocytochemistry, ICC (1:200-1:500)

Purification

Protein G purified

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

References & Citations

Bhasin M, Reinherz EL, Reche PA (2006) . "Recognition and classification of histones using support vector machine". J. Comput. Biol. 13 (1) : 102–12. Fischle, W., Tseng, B. S., Dormann, H. L., Ueberheide, B. M., Garcia, B. A., Shabanowitz, J., Hunt, D. F., Funabiki, H., Allis, C. D. Regulation of HP1-chromatin binding by histone H3 methylation and phosphorylation. Nature 438: 1116-1122, 2005.