Monoclonal Anti- Synaptophysin antibody

Synaptophysin, also known as the major synaptic vesicle protein p38, is an integral membrane protein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass, accounting for about 10% of total vesicle protein.1The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses.2

Western Blot, WB (1:100-1:500) ; Immunohistochemistry, IHC-P (1:20-1:100) ; Immunocytochemistry, ICC (1:20-1:100) ; Flow Cytometry, Flow-Cyt (1:20-1:50)

Kwon, S. E., Chapman, E. R. Synaptophysin regulates the kinetics of synaptic vesicle endocytosis in central neurons. Neuron 70: 847-854, 2011. Calhoun ME, Jucker M, Martin LJ, Thinakaran G, Price DL, Mouton PR (December 1996) . "Comparative evaluation of synaptophysin-based methods for quantification of synapses". J. Neurocytol. 25 (12) : 821–8.