Monoclonal Anti- Proliferating Cell Nuclear Antigen, PCNA antibody

Product Specifications

Background

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. [1]This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. [2]It induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. And It has to be loaded onto DNA in order to be able to stimulate APEX2. [3]

Host

Mouse

Reactivity

Human, mouse, rat

Immunogen

Recombinant PCNA protein.

Clone

M65-8

Applications

Western Blot, WB (1:500-1:1, 000) ; Immunohistochemistry, IHC-P (1:100) ; Flow Cytometry, Flow-Cyt (1:1:50)

Purification

Protein A purified

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

References & Citations

1. Bowman GD, O'Donnell M, Kuriyan J (2004) . "Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex". Nature 429 (6993) : 724–730. 2. Burkovics P., Hajdu I., Szukacsov V., Unk I., Haracska L."Role of PCNA-dependent stimulation of 3'-phosphodiesterase and 3'-5' exonuclease activities of human Ape2 in repair of oxidative DNA damage." Nucleic Acids Res. 37:4247-4255 (2009) 3. Motegi A., Liaw H.-J., Lee K.-Y., Roest H.P., Maas A., Wu X., Moinova H., Markowitz S.D., Ding H., Hoeijmakers J.H.J., Myung K. "Polyubiquitination of proliferating cell nuclear antigen by HLTF and SHPRH prevents genomic instability from stalled replication forks." Proc. Natl. Acad. Sci. U.S.A. 105:12411-12416 (2008)