Monoclonal Anti- Chromogranin-A, CgA antibody

Product Specifications

Background

Chromogranin A (CgA), also calledparathyroid secretory protein 1, is a member of theHYPERLINK "http://en.wikipedia.org/wiki/Granin" \o "Granin"graninfamily of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells.Chromogranin A gene has 8 exons and 7 introns spanning about 11 kb1. Itis elevated inHYPERLINK "http://en.wikipedia.org/wiki/Pheochromocytoma" \o "Pheochromocytoma"pheochromocytomas2, and also used as an indicator for pancreas and prostate cancer3and in carcinoid syndrome4. It alsoplaysa role in early neoplasic progression.

Host

Mouse

Reactivity

Human, mouse, rat

Immunogen

A synthetic peptide (conjugated with KLH) corresponding to the C-terminal of human CgA.

Clone

S27-1

Applications

Western Blot, WB (1:100-1:200) ; Immunohistochemistry, IHC-P (1:20-1:100) ; Immunocytochemistry, ICC (1:20-1:100)

Purification

Cultured cell supernatant, Protein A purified

Form

Liquid

Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol and 0.05% Sodium Azide.

References & Citations

1.Wu, H.-J., Rozansky, D. J., Parmer, R. J., Gill, B. M., O'Connor, D. T. Structure and function of the chromogranin A gene: clues to evolution and tissue-specific expression. J. Biol. Chem. 266: 13130-13134, 1991. 2.Cotesta D, Caliumi C, Alò P, Petramala L, Reale MG, Masciangelo R, Signore A, Cianci R, D'Erasmo E, Letizia C (2005) . "High plasma levels of human chromogranin A and adrenomedullin in patients with pheochromocytoma".Tumori91 (1) : 53–8. 3.Wu JT, Erickson AJ, Tsao KC, Wu TL, Sun CF (April 2000) . "Elevated serum chromogranin A is detectable in patients with carcinomas at advanced disease stages".Ann. Clin. Lab. Sci.30 (2) : 175–8. 4.Nikou GC, Lygidakis NJ, Toubanakis C, Pavlatos S, Safioleas M (2005) . "Current diagnosis and treatment of gastrointestinal carcinoids in a series of 101 patients: the significance of serum chromogranin-A, somatostatin receptor scintigraphy and somatostatin analogues".Hepatogastroenterology52 (63) : 731–41.