Mouse Tumor Necrosis Factor-alpha, TNF-α HiLA Kit

Incubate the acceptor beads coated with anti-TNF-α antibody 1, biotin conjugated anti- TNF-α antibody 2, and the sample to form a sandwich immune complex (antibody 1-sample- antibody 2) . the immune complex is the n incubated withdonor beads coated with streptavid into form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration of TNF-α in the sample is calculated through mathe matical fitting. In the absence of human TNF-α in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.