Human Interleukin-10, IL-10 HiLA Kit

Incubate the Fconjugated anti-IL-10 antibody 1, biotin conjugated anti-IL-10 antibody 2, acceptor beads coated with F-conjugate, and the sample to form a sandwich immune complex (antibody 1-sample- antibody 2) . the immune complex is the n incubated withdonor beads coated with streptavid into form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the receptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration ofIL-10in the sample is calculated through mathe matical fitting. In the absence of humanIL-10in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the receptor beads do not emit any light.