Human Interleukin-2, IL-2 HiLA Kit

Interleukin-2 (IL-2) is an interleukin, which is a type of cytokine signaling molecule forming part of the immune system. It is a 15.5–16 kDa proteinthat regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes. The major sources of IL-2 are activated CD4+ T cells and activated CD8+ T cells.Put shortly the function of IL-2 is to stimulate the growth of helper, cytotoxic and regulatory T cells.

Product Specifications

Applications

For quantitative detection of human IL-2 in buffer solution, serum, cell culture supernatant.

Assay Principle

Incubate the acceptor beads coated with anti-IL2 antibody1, biotin conjugated anti-IL-2 antibody 2, and the sample to form a sandwich immune complex (antibody 1-sample- antibody 2) . the immune complex is the n incubated withdonor beads coated with streptavidin to form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration ofIL-2in the sample is calculated through mathe matical fitting. In the absence of humanIL-2in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.