Human Interleukin-1β, IL-1β HiLA Kit
Interleukin-1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene.There are two genes for interleukin-1 (IL-1) : IL-1 alpha and IL-1 beta (this gene) . IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β.
Product Specifications
Applications
For quantitative detection of human IL-1β in buffer solution, serum, cell culture supernatant.
Assay Principle
Incubate the Fconjugated anti- IL-1β antibody 1, biotin conjugated anti- IL-1β antibody 2, acceptor beads coated with F-conjugate, and the sample to form a sandwich immune complex (antibody 1-sample- antibody 2) . the immune complex is the n incubated withdonor beads coated with streptavid into form a chemiluminescent complex, ensuring that the distance between the two beads is less than 200 nm. When the distance between the two beads is less than 200 nm, the donor beads is excited by light (680 nm), producing singlet oxygen which diffuses to the acceptor beads, inducing energy transfer. the acceptor beads absorb this energy and emit scattering light at 615 nm. the light signal is collected by a photosensitive detector, and the concentration of IL-1β in the sample is calculated through mathe matical fitting. In the absence of human IL-1β in the sample, no immune complex is formed, or the distance between the two beads exceeds 200 nm, exceeding the transfer distance for singlet oxygen, and the acceptor beads do not emit any light.
Detection Range
0 pg/ml - 30,000 pg/ml
Linearity
0.32 pg/ml - 3,000 pg/ml
Storage Conditions
Store at 2-8°C for 6 months.
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