Recombinant Human FABP3 Protein

Recombinant human FABP3 is produced by the E.coli system, with the target gene encoding Val2-Ala133 expressedwith 6x His-tag at the C-terminus.

Product Specifications

Background

Heart-type fatty acid binding protein (hFABP) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. Heart-type Fatty Acid-Binding Protein (H-FABP) is a small cytoplasmic protein (15 kDa) released from cardiac myocytes following an ischemic episode. Like the nine other distinct FABPs that have been identified, H-FABP is involved in active fatty acid metabolism where it transports fatty acids from the cell membrane to mitochondria for oxidation. See FABP3 for biochemical details.

Synonyms

FABP3, FABP11, H-FABP, M-FABP, MDGI, O-FABP, Heart-type fatty acid binding protein, fatty acid binding protein 3

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<1 EU/µg by LAL test

Purity

>95%by SDS-PAGE

Buffer

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 5% Trehalose, 5% Mannitol, 0.02% Tween 80, pH 6.0.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)

References & Citations

Phelan CM, Larsson C, Baird S, Futreal PA, Ruttledge MH, Morgan K, Tonin P, Hung H, Korneluk RG, Pollak MN, Narod SA (May 1996) . "The human mammary-derived growth inhibitor (MDGI) gene: genomic structure and mutation analysis in human breast tumors". Genomics. 34 (1) : 63–8. Kleine AH, Glatz JF, Van Nieuwenhoven FA, Van der Vusse GJ (Oct 1992) . "Release of heart fatty acid-binding protein into plasma after acute myocardial infarction in man". Molecular and Cellular Biochemistry. 116 (1–2) : 155–62.