Recombinant Human CNTF Protein
Recombinant human CNTF is produced by the E.coli system, with the target gene encoding Ala2-Met200 expressed.
Product Specifications
Background
Ciliary neurotrophic factor is a protein that in humans is encoded by the CNTF gene. The protein encoded by this gene is a polypeptide hormone and neurotrophic factor whose actions have mainly been studied in the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neural populations including astrocytes. It is a hypothalamic neuropeptide that is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also cotranscribed with the upstream ZFP91 gene.
Synonyms
CNTF, Hciliary neurotrophic factor
Source
E.coli
Applications
SDS-PAGE
Endotoxin
<1 EU/µg by LAL test
Purity
>95%by SDS-PAGE
Buffer
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 8.0.
Reconstitution
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)
References & Citations
Lam A, Fuller F, Miller J, Kloss J, Manthorpe M, Varon S, Cordell B (Sep 1991) . "Sequence and structural organization of the human gene encoding ciliary neurotrophic factor". Gene. 102 (2) : 271–6. Bazan JF (Sep 1991) . "Neuropoietic cytokines in the hematopoietic fold". Neuron. 7 (2) : 197–208.
Storage Conditions
Shipat 4°C. Upon receipt, aliquot and store at -20°Cor -80°C for long term. Avoid repeated freeze and thaw cycles.
Formulation
Lyophilized
Uniprot Accession Number
P26441
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
