Recombinant Human IL-1RA Protein
Recombinant Human IL-1RA is produced by the E.coli system, with the target gene encoding Arg26-Glu177 expressed.
Product Specifications
Background
The interleukin-1 receptor antagonist (IL-1RA) is a protein that in humans is encoded by the IL1RN gene.IL-1RA was initially called the IL-1 inhibitor and was discovered separately in 1984 by two independent laboratories.IL-1RA is an agent that binds non-productively to the cell surface interleukin-1 receptor (IL-1R), the same receptor that binds interleukin 1 family (IL-1), preventing IL-1's from sending a signal to that cell.
Synonyms
IL1RN, DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4, interleukin 1 receptor antagonist
Source
E.coli
Applications
SDS-PAGE
Endotoxin
<1 EU/µg by LAL test
Purity
>95%by SDS-PAGE
Buffer
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 8% Trehalose, 4% Mannitol, 50mM NaCl, 0.05% Tween80, pH7.5.
Reconstitution
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)
References & Citations
Steinkasserer A, Spurr NK, Cox S, Jeggo P, Sim RB (July 1992) . "The human IL-1 receptor antagonist gene (IL1RN) maps to chromosome 2q14-q21, in the region of the IL-1 alpha and IL-1 beta loci". Genomics. 13 (3) : 654–657. Patterson D, Jones C, Hart I, Bleskan J, Berger R, Geyer D, et al. (January 1993) . "The human interleukin-1 receptor antagonist (IL1RN) gene is located in the chromosome 2q14 region". Genomics. 15 (1) : 173–176. Dinarello CA (December 1994) . "The interleukin-1 family: 10 years of discovery". FASEB Journal. 8 (15) : 1314–1325.
Storage Conditions
Shipat 4°C. Upon receipt, aliquot and store at -20°Cor -80°C for long term. Avoid repeated freeze and thaw cycles.
Formulation
Lyophilized
Uniprot Accession Number
P18510
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