Recombinant Mouse IL-1b Protein

Recombinant mouse IL-1b is produced by the E.coli system, with the target gene encoding Val118-Ser269 expressed.

Product Specifications

Background

Interleukin-1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene.There are two genes for interleukin-1 (IL-1) : IL-1 alpha and IL-1 beta (this gene) . IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β.

Synonyms

IL1B, IL-1, IL1-BETA, IL1F2, interleukin 1 beta, IL1beta

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<0.01 EU/µg by LAL test

Purity

>95%by SDS-PAGE

Buffer

Lyophilized from a 0.2 μm filtered solution of 50mM Tris-HCl,50mM NaCl,4%Mannitol, pH 8.0.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)

References & Citations

Auron PE, Webb AC, Rosenwasser LJ, Mucci SF, Rich A, Wolff SM, Dinarello CA (December 1984) . "Nucleotide sequence of human monocyte interleukin 1 precursor cDNA". Proceedings of the National Academy of Sciences of the United States of America. 81 (24) : 7907–7911. "Catabolin" is the name given by Jeremy Saklatvala for IL-1 alpha. March CJ, Mosley B, Larsen A, Cerretti DP, Braedt G, Price V, et al. (1985) . "Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs". Nature. 315 (6021) : 641–647. Clark BD, Collins KL, Gandy MS, Webb AC, Auron PE (October 1986) . "Genomic sequence for human prointerleukin 1 beta: possible evolution from a reverse transcribed prointerleukin 1 alpha gene". Nucleic Acids Research. 14 (20) : 7897–7914. Bensi G, Raugei G, Palla E, Carinci V, Tornese Buonamassa D, Melli M (1987) . "Human interleukin-1 beta gene". Gene. 52 (1) : 95–101.