Recombinant Human IL-17A Protein

Recombinant human IL-17A is produced by the E.coli system, with the target gene encoding Gly24-Ala155 expressed.

Product Specifications

Background

Interleukin-17A is a protein that in humans is encoded by the IL17A gene. In rodents, IL-17A used to be referred to as CTLA8, after the similarity with a viral gene (O40633) .The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO) .

Synonyms

IL17A, CTLA8, IL-17, IL-17A, IL17, CTLA-8, interleukin 17A, ILA17, Interleukin 17A

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<1 EU/µg by LAL test

Purity

>95%by SDS-PAGE

Buffer

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)

References & Citations

Rouvier E, Luciani MF, Mattéi MG, Denizot F, Golstein P (June 1993) . "CTLA-8, cloned from an activated T cell, bearing AU-rich messenger RNA instability sequences, and homologous to a herpesvirus saimiri gene". Journal of Immunology. 150 (12) : 5445–5456. Yao Z, Painter SL, Fanslow WC, Ulrich D, Macduff BM, Spriggs MK, Armitage RJ (December 1995) . "Human IL-17: a novel cytokine derived from T cells". Journal of Immunology. 155 (12) : 5483–5486.