Recombinant Human Beta-NGF Protein
Recombinant human Beta-NGF is produced by the E.coli system, with the target gene encoding Ser122-Ala241 expressed.
Product Specifications
Background
β-NGF, also known as mature or functional NGF, is produced after degradation of the signaling peptide and proteolytic cleavage of pro-NGF at Ser122. β-NGF homodimerizes and induces neurotropic signaling through tropomyosin-related kinase A (TrkA) and the p75 neurotrophin receptor (p75NTR) .
Synonyms
Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB, β-NGF
Source
E.coli
Applications
SDS-PAGE
Endotoxin
<0.01 EU/µg by LAL test
Purity
>95%by SDS-PAGE
Buffer
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)
References & Citations
Freeman RS, Burch RL, Crowder RJ, Lomb DJ, Schoell MC, Straub JA, et al. (2004) . "NGF deprivation-induced gene expression: After ten years, where do we stand". NGF and Related Molecules in Health and Disease. Progress in Brain Research. Vol. 146. pp. 111–26. Ratto MH, Leduc YA, Valderrama XP, van Straaten KE, Delbaere LT, Pierson RA, et al. (September 2012) . "The nerve of ovulation-inducing factor in semen". Proceedings of the National Academy of Sciences of the United States of America. 109 (37) : 15042–15047.
Storage Conditions
Shipat 4°C. Upon receipt, aliquot and store at -20°Cor -80°C for long term. Avoid repeated freeze and thaw cycles.
Formulation
Lyophilized
Uniprot Accession Number
P01138
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