Recombinant Human Beta-NGF Protein

Recombinant human Beta-NGF is produced by the E.coli system, with the target gene encoding Ser122-Ala241 expressed.

Product Specifications

Background

β-NGF, also known as mature or functional NGF, is produced after degradation of the signaling peptide and proteolytic cleavage of pro-NGF at Ser122. β-NGF homodimerizes and induces neurotropic signaling through tropomyosin-related kinase A (TrkA) and the p75 neurotrophin receptor (p75NTR) .

Synonyms

Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB, β-NGF

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<0.01 EU/µg by LAL test

Purity

>95%by SDS-PAGE

Buffer

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)

References & Citations

Freeman RS, Burch RL, Crowder RJ, Lomb DJ, Schoell MC, Straub JA, et al. (2004) . "NGF deprivation-induced gene expression: After ten years, where do we stand". NGF and Related Molecules in Health and Disease. Progress in Brain Research. Vol. 146. pp. 111–26. Ratto MH, Leduc YA, Valderrama XP, van Straaten KE, Delbaere LT, Pierson RA, et al. (September 2012) . "The nerve of ovulation-inducing factor in semen". Proceedings of the National Academy of Sciences of the United States of America. 109 (37) : 15042–15047.