Recombinant Human pro-BDNF Protein

Recombinant human pro-BDNF is produced by the E.coli system, with the target gene encoding Ala19- Arg247 (R125A, R127A, R128A) expressed.

Product Specifications

Background

Brain-derived neurotrophic factors (Bdnf), or abrineurin, is a proteinthat, in humans, is encoded by the BDNF gene.BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor (NGF), a family which also includes NT-3 and NT-4/NT-5. Neurotrophic factors are found in the brain and the periphery. BDNF was first isolated from a pig brain in 1982 by Yves-Alain Barde and Hans Thoenen.BDNF activates the TrkB tyrosine kinase receptor.

Synonyms

ANON2, BULN2, BDNF, brain-derived neurotrophic factor, brain derived neurotrophic factor

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<1 EU/µg by LAL test

Purity

>95%by SDS-PAGE

Buffer

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl,10% Trehalose,10% Glycerol,200mM NaCl,1mM EDTA,0.05% Tween80, pH 7.8.

References & Citations

Binder DK, Scharfman HE (September 2004) . "Brain-derived neurotrophic factor". Growth Factors. 22 (3) : 123–31. Jones KR, Reichardt LF (October 1990) . "Molecular cloning of a human gene that is a member of the nerve growth factor family". Proceedings of the National Academy of Sciences of the United States of America. 87 (20) : 8060–64. Maisonpierre PC, Le Beau MM, Espinosa R, Ip NY, Belluscio L, de la Monte SM, et al. (July 1991) . "Human and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations". Genomics. 10 (3) : 558–68. Kowiański P, Lietzau G, Czuba E, Waśkow M, Steliga A, Moryś J (April 2018) . "BDNF: A Key Factor with Multipotent Impact on Brain Signaling and Synaptic Plasticity". Cellular and Molecular Neurobiology. 38 (3) : 579–593.