Recombinant Human Leptin Protein

Recombinant human leptin is produced by the E.coli system, with the target gene encoding Val22-Cys167 expressed.

Product Specifications

Background

Leptin, also known as obese protein, is a protein hormone predominantly made by adipocytes (cells of adipose tissue) . Its primary role is likely to regulate long-term energy balance.As one of the major signals of energy status, leptin levels influence appetite, satiety, and motivated behaviors oriented toward the maintenance of energy reserves (e.g., feeding, foraging behaviors) .The amount of circulating leptin correlates with the amount of energy reserves, mainly triglycerides stored in adipose tissue. High leptin levels are interpreted by the brain that energy reserves are high, whereas low leptin levels indicate that energy reserves are low, in the process adapting the organism to starvation through a variety of metabolic, endocrine, neurobiochemical, and behavioral changes.

Synonyms

LEP, LEPD, OB, OBS, leptin

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<1 EU/µg by LAL test

Purity

>95%by SDS-PAGE

Buffer

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl,50mM NaCl,10%Trehalose,0.02%Tween80, pH8.5.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)

References & Citations

Al-Hussaniy HA, Alburghaif AH, Naji MA (2021) . "Leptin hormone and its effectiveness in reproduction, metabolism, immunity, diabetes, hopes and ambitions". Journal of Medicine and Life. 14 (5) : 600–605. Hebebrand J, Hildebrandt T, Schlögl H, Seitz J, Denecke S, Vieira D, et al. (October 2022) . "The role of hypoleptinemia in the psychological and behavioral adaptation to starvation: Implications for anorexia nervosa". Neuroscience and Biobehavioral Reviews. 141 104807.