Recombinant Human IGF-1 Protein
Recombinant human IGF-1 is produced by the E.coli system, with the target gene encoding Gly49-Ala118 expressed.
Product Specifications
Background
Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a hormone similar in molecular structure to insulin which plays an important role in childhood growth, and has anabolic effects in adults.In the 1950s IGF-1 was called "sulfation factor" because it stimulated sulfation of cartilage in vitro, and in the 1970s due to its effects it was termed "nonsuppressible insulin-like activity" (NSILA) .
Synonyms
IGF1, IGF-I, IGF1A, IGFI, MGF, insulin like growth factor 1, IGF
Source
E.coli
Applications
SDS-PAGE
Endotoxin
<0.5 EU/µg by LAL test
Purity
>95%by SDS-PAGE
Buffer
Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5
Reconstitution
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)
References & Citations
Tahimic CG, Wang Y, Bikle DD (2013) . "Anabolic effects of IGF-1 signaling on the skeleton". Frontiers in Endocrinology. 4: 6. Salmon WD, Daughaday WH (June 1957) . "A hormonally controlled serum factor which stimulates sulfate incorporation by cartilage in vitro". The Journal of Laboratory and Clinical Medicine. 49 (6) : 825–836.
Storage Conditions
Shipat 4°C. Upon receipt, aliquot and store at -20°Cor -80°C for long term. Avoid repeated freeze and thaw cycles.
Formulation
Lyophilized
Uniprot Accession Number
P05019
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