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Recombinant Human CCL2 Protein

Recombinant human CCL2 is produced by the E.coli system, with the target gene encoding Gln24-Thr99 expressed.

Product Specifications

Background

The chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemoattractant protein 1 (MCP1) and small inducible cytokine A2. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 tightly regulates cellular mechanicsand thereby recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection.

Synonyms

CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF, C-C motif chemokine ligand 2

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<1 EU/µg by LAL test

Purity

>95%by SDS-PAGE

Buffer

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH6.0.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)

References & Citations

Evers TM, Sheikhhassani V, Haks MC, Storm C, Ottenhoff TH, Mashaghi A (January 2022) . "Single-cell analysis reveals chemokine-mediated differential regulation of monocyte mechanics". iScience. 25 (1) 103555. Carr MW, Roth SJ, Luther E, Rose SS, Springer TA (April 1994) . "Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant". Proceedings of the National Academy of Sciences of the United States of America. 91 (9) : 3652–6. Xu LL, Warren MK, Rose WL, Gong W, Wang JM (September 1996) . "Human recombinant monocyte chemotactic protein and other C-C chemokines bind and induce directional migration of dendritic cells in vitro". Journal of Leukocyte Biology. 60 (3) : 365–71.

Storage Conditions

Shipat 4°C. Upon receipt, aliquot and store at -20°Cor -80°C for long term. Avoid repeated freeze and thaw cycles.

Formulation

Lyophilized

Uniprot Accession Number

P13500

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