Recombinant Human CALM1 Protein

Recombinant human Calmodulin is produced by the E.coli system, with the target gene encoding Met1-Lys149 expressed.

Product Specifications

Background

Calmodulin (CaM) (an abbreviation for calcium-modulated protein) is a multifunctional intermediate calcium-binding messenger protein expressed in all eukaryotic cells.It is an intracellular target of the secondary messenger Ca2+, and the binding of Ca2+ is required for the activation of calmodulin. Once bound to Ca2+, calmodulin acts as part of a calcium signal transduction pathway by modifying its interactions with various target proteins such as kinases or phosphatases.

Synonyms

Calmodulin, CaM, CALM1, CALM, CAM, CAM1, CALM2, CAM2, CAMB, CALM3, CALML2, CAM3, CAMC, CAMIII

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<1 EU/µg by LAL test

Purity

>95%by SDS-PAGE

Buffer

Lyophilized from a 0.2 μm filtered solution of 50mM NH4HCO3, pH 8.0.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)

References & Citations

Stevens FC (August 1983) . "Calmodulin: an introduction". Canadian Journal of Biochemistry and Cell Biology. 61 (8) : 906–10. Chin D, Means AR (August 2000) . "Calmodulin: a prototypical calcium sensor". Trends in Cell Biology. 10 (8) : 322–8. Purves D, Augustine G, FitzpatrickD, Hall W, LaMantia AS, White L (2012) . Neuroscience. Massachusetts: Sinauer Associates. pp. 95, 147, 148.