Recombinant Human ANG Protein

Recombinant human ANG is produced by the E.coli system, with the target gene encoding Gln25-Pro147 expressed.

Product Specifications

Background

Angiogenin (ANG) also known as ribonuclease 5 is a small 123 amino acid protein that in humans is encoded by the ANG gene.Angiogenin is a potent stimulator of new blood vessels through the process of angiogenesis. Ang hydrolyzes cellular RNA, resulting in modulated levels of protein synthesis and interacts with DNA causing a promoter-like increase in the expression of rRNA.Ang is associated with cancer and neurological disease through angiogenesis and through activating gene expression that suppresses apoptosis.

Synonyms

ANG, ALS9, HEL168, RAA1, RNASE4, RNase A family 5, RNASE5, angiogenin, ribonuclease, angiogenin

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<1 EU/µg by LAL test

Purity

>95%by SDS-PAGE

Buffer

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)

References & Citations

Tello-Montoliu A., Patel J.V., Lip G.Y.H. (2006) . "Angiogenin: a review of the pathophysiology and potential clinical applications". Journal of Thrombosis and Haemostasis. 4 (9) : 1864–74. Xu Z, Tsuji T, Riordan J, Hu G (2003) . "Identification and characterization of an angiogenin-binding DNA sequence that stimulate luciferase reporter gene expression". Biochemistry. 42 (1) : 121–128. Li S, Yu W, Hu GF (2012) . "Angiogenin inhibits nuclear translocation of apoptosis inducing factor in a Bcl-2-dependent manner". Journal of Cellular Physiology. 227 (4) : 1639–1644. Steidinger TU, Standaert DG, Yacoubian TA (2011) . "A neuroprotective role for angiogenin in models of Parkinson's disease". Journal of Neurochemistry. 116 (3) : 334–341.