Recombinant Human GDNF Protein

Recombinant human GDNF is produced by the E.coli system, with the target gene encoding Ser78-Ile211 expressed.

Product Specifications

Background

Glial cell line-derived neurotrophic factor (GDNF) is a protein that, in humans, is encoded by the GDNF gene.GDNF is a small protein that potently promotes the survival of many types of neurons.It signals through GFRα receptors, particularly GFRα1. It is also responsible for the determination of spermatogonia into primary spermatocytes, i.e. it is received by RET proto-oncogene (RET) and by forming gradient with SCF it divides the spermatogonia into two cells. As the result there is retention of spermatogonia and formation of spermatocyte.

Synonyms

GDNF, ATF1, ATF2, glial cell derived neurotrophic factor, HFB1-HSCR3, ATF

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<1 EU/µg by LAL test

Purity

>95%by SDS-PAGE

Buffer

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)

References & Citations

Lin LF, Doherty DH, Lile JD, Bektesh S, Collins F (May 1993) . "GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons". Science. 260 (5111) . New York, N.Y.:1130–1132.