Recombinant Human EGF Protein
Recombinant human EGF is produced by the E.coli system, with the target gene encoding Asn971-Arg1023 expressed.
Product Specifications
Background
Epidermal growth factor (EGF) is a protein that stimulates cell growth and differentiation by binding to its receptor, EGFR. Human EGF is 6-kDaand has 53 amino acid residues and three intramolecular disulfide bonds.EGF was originally described as a secreted peptide found in the submaxillary glands of mice and in human urine. EGF has since been found in many human tissues, including platelets, submandibular gland (submaxillary gland), and parotid gland.Initially, human EGF was known as urogastrone.
Synonyms
Epidermal growth factor, EGF, HOMG4, URG, epithelial growth factor
Source
E.coli
Applications
SDS-PAGE
Endotoxin
<0.01EU/µg by LAL test
Purity
>95%by SDS-PAGE
Buffer
Lyophilized from a 0.2 μm filtered solution of 20mM Tris,200mMNaCl,4%Mannitol, pH 8.0.
Reconstitution
Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration<100μg/ml.)
References & Citations
Harris RC, Chung E, Coffey RJ (March 2003) . "EGF receptor ligands". Experimental Cell Research. 284 (1) : 2–13. Carpenter G, Cohen S (May 1990) . "Epidermal growth factor". The Journal of Biological Chemistry. 265 (14) : 7709–12. Custo S, Baron B, Felice A, Seria E (5 July 2022) . "A comparative profile of total protein and six angiogenically-active growth factors in three platelet products". GMS Interdisciplinary Plastic and Reconstructive Surgery DGPW. 11 (Doc06) : Doc06. Venturi S, Venturi M (2009) . "Iodine in evolution of salivary glands and in oral health". Nutrition and Health. 20 (2) : 119–34. Hollenberg MD, Gregory H (May 1980) . "Epidermal growth factor-urogastrone: biological activity and receptor binding of derivatives". Molecular Pharmacology. 17 (3) : 314–320.
Storage Conditions
Shipat 4°C. Upon receipt, aliquot and store at -20°Cor -80°C for long term. Avoid repeated freeze and thaw cycles.
Formulation
Lyophilized
Uniprot Accession Number
P01133
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
