Recombinant Human /Mouse/Rat BDNF Protein

Recombinant human BDNF is produced by the E.coli system, with the target gene encoding His129-Arg247 expressed.

Product Specifications

Background

Brain-derived neurotrophic factor, also known as BDNF, is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. BDNF is a member of the "neurotrophin" family of growth factors, which are related to the NGF. It acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain—areas vital to learning, memory, and higher thinking. Post mortem analysis has shown lowered levels of BDNF in the brain tissues of people with Alzheimer's disease.

Synonyms

Brain-derived neurotrophic factor, BDNF, brain-derived neurotrophic factor, ANON2, BULN2, brain derived neurotrophic factor

Source

E.coli

Applications

SDS-PAGE

Endotoxin

<0.01EU/µg by LAL test

Purity

>95% by SDS-PAGE

Buffer

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 400mM NaCl, pH 7.2.

Reconstitution

Dissolve the lyophilized protein in distilled water (It is not recommended to reconstitute to a concentration <100μg/ml.)

References & Citations

Binder DK, Scharfman HE (September 2004) . "Brain-derived Neurotrophic Factor". Growth Factors 22 (3) : 123–31. Maisonpierre PC, Le Beau MM, Espinosa R et al. (July 1991) . "Rabbit and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations". Genomics 10 (3) : 558–68. Maisonpierre PC, Le Beau MM, Espinosa R et al. (July 1991) . "Rabbit and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations". Genomics 10 (3) : 558–68.