Recombinant Human IGFBP4 Protein

Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4 gene.IGFBP4 maternal blood concentrations were lower during pregnancy than during non-pregnant cycles and synchronized control cycles. It can be speculated that the lower IGFBP4 in maternal blood may result in an increase of free IGF1 for local action. IGFBP4 downregulation in GCTs compared to MSCs. Overexpression of UCHL1 and IGFBP4 by stable transfection of GCTSC did not influence cell viability, proliferation, migration and chemosensitivity compared to parental cells. However, colony-formation was significantly decreased suggesting that rescue of UCHL1 and IFGBP4 suppresses clonogenicity of GCT stromal cells. Epigenetic silencing of UCHL1 and IGFBP4 in GCTs might thus be a crucial event during the malignant transformation of MSCs in the context of GCT development and represent promising targets for the development of new diagnostic and therapeutic strategies.

Meyerholz MM, Mense K, Lietzau M, Kassens A, Linden M, Knaack H, Wirthgen E, Hoeflich A, Raliou M, Richard C, Sandra O, Schuberth HJ, Hoedemaker M, Schmicke M. Serum IGFBP4 concentration decreased in dairy heifers towards day 18 of pregnancy. J Vet Sci. 2015;16 (4) :413-21. Fellenberg J, Sähr H, Liu L, Schönsiegel F, Herr I. Rescue of silenced UCHL1 and IGFBP4 expression suppresses clonogenicity of giant cell tumor-derived stromal cells. Cancer Lett. 2013 Aug 9;336 (1) :61-7.